Indirect ultraviolet detection of biologically relevant organic acids by capillary electrophoresis
نویسندگان
چکیده
منابع مشابه
Rapid determination of nonaromatic organic acids in honey by capillary zone electrophoresis with direct ultraviolet detection.
A rapid capillary zone electrophoresis (CZE) method with direct ultraviolet (UV) detection has been set up and developed to determine the most important nonaromatic organic acids in honey with a really simple treatment of the sample. The determination of oxalic, formic, malic, succinic, pyruvic, acetic, lactic, citric, and gluconic acids has been carried out in 4 min. The electrolyte compositio...
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Capillary electrophoresis (CE) with indirect UV detection is an interesting analytical method for the analysis of drugs and pharmaceuticals. Good and reproducible capillary quality is needed to develop robust methods and to facilitate method transfer in CE. It is widely accepted that preconditioning procedures are indispensable in capillary electrophoresis in order to achieve reproducibility of...
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Capillary electrophoresis (CE) with indirect UV detection is an interesting analytical method for the analysis of drugs and pharmaceuticals. Good and reproducible capillary quality is needed to develop robust methods and to facilitate method transfer in CE. It is widely accepted that preconditioning procedures are indispensable in capillary electrophoresis in order to achieve reproducibility of...
متن کاملComputer Simulation of Capillary Electrophoresis - Analysis of Organic Acids
a) Run the program HPCESIM. Copy all basic parameters (Equipment, Capillary, Buffer, Sample, Inject and Detect) for electrophoretic separation of mixture of 4 organic acids into table 1. Table 1 Voltage U (kV) Current I (μA) Thermostating temperature T (oC) Capillary length to detector L (mm) ID (inner diameter of capillary) (μm) Separation mode (anionic/cationic) Composition of basic electroly...
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We describe a method for measuring urinary oxalate and citrate with capillary electrophoresis (CE) and indirect ultraviolet absorbance detection. Sample preparation is minimal, requiring an acidification, brief centrifugation, and dilution. The method is rapid, with oxalate and citrate having mean migration times of 4.02 and 4.50 min, respectively. The minimal detectable concentration (signal-t...
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ژورنال
عنوان ژورنال: Journal of Chromatography B: Biomedical Sciences and Applications
سال: 1996
ISSN: 0378-4347
DOI: 10.1016/0378-4347(96)00002-3